(26-01-14) Novel regulatory roles of omega-3 fatty acids in metabolic pathways: a proteomics approach

Abeer A Ahmed, Kayode A Balogun, Natalia V Bykova and Sukhinder K Cheema


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Abstract (provisional)
Background

Omega-3 polyunsaturated fatty acids (n-3 PUFA) have been shown to alleviate
the symptoms of metabolic disorders, such as heart disease, diabetes,
obesity and insulin resistance. Several putative mechanisms by which n-3
PUFA elicit beneficial health effects have been proposed; however, there is
still a shortage of knowledge on the proteins and pathways that are
regulated by n-3 PUFA.
Methods

Using two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and
liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we
investigated the effects of diets high or low in n-3 PUFA on hepatic
proteomic profile of C57BL/6 mice.
Results

The findings show for the first time that high dietary n-3 PUFA reduced the
expression of regucalcin, adenosine kinase and aldehyde dehydrogenase. On
the other hand, diets high in n-3 PUFA increased the expression of
apolipoprotein A-I, S-adenosylmethionine synthase, fructose-1,
6-bisphosphatase, ketohexokinase, malate dehydrogenase, GTP-specific
succinyl CoA synthase, ornithine aminotransferase and protein disulfide
isomerase-A3.
Conclusions

Our findings revealed for the first time that n-3 PUFA causes alterations in
several novel functional proteins involved in regulating lipid,
carbohydrate, one-carbon, citric acid cycle and protein metabolism,
suggesting integrated regulation of metabolic pathways. These novel proteins
are potential targets to develop therapeutic strategies against metabolic
disorders.


Source: Nutrition & Metabolism 2014, 11:6 doi:10.1186/1743-7075-11-6
Published: 17 January 2014

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