(15-02-11) The Effect of Very-Low-Calorie Diet on mRNA Expression of Inflammation-Related Genes in Subcutaneous Adipose Tissue and Peripheral M

The Effect of Very-Low-Calorie Diet on mRNA Expression of Inflammation-Related Genes in Subcutaneous Adipose Tissue and Peripheral Monocytes of Obese Patients with Type 2 Diabetes Mellitus.

Mraz M, Lacinova Z, Drapalova J, Haluzikova D, Horinek A, Matoulek M, Trachta
P, Kavalkova P, Svacina S, Haluzik M.

Third Department of Medicine (M.Mr., Z.L., J.D., D.H., A.H., M.Ma., P.T., P.
K., S.S., M.H.) and Department of Sports Medicine (D.H.), General University
Hospital and First Medical Faculty, Charles University, 128 08 Prague 2, Czech
Republic.
Abstract
Context: Low-grade inflammation links obesity, type 2 diabetes mellitus
(T2DM), and cardiovascular diseases. Objective: To explore the expression
profile of genes involved in inflammatory pathways in adipose tissue and
peripheral monocytes (PM) of obese patients with and without T2DM at baseline
and after dietary intervention. Design: Two-week intervention study with very-
low-calorie diet (VLCD). Setting: University hospital. Patients: Twelve obese
females with T2DM, 8 obese nondiabetic females (OB) and 15 healthy age-matched
females. Intervention: Two weeks of VLCD (2500 kJ/d). Main Outcome Measures:
Metabolic parameters, circulating cytokines, hormones, and mRNA expression of
39 genes in sc adipose tissue (SCAT) and PM. Results: Both T2DM and OB group
had significantly increased serum concentrations of circulating proinflammatory
factors (C-reactive protein, TNF?, IL-6, IL-8), mRNA expression of macrophage
antigen CD68 and proinflammatory chemokines (CCL-2, -3, -7, -8, -17, -22) in
SCAT and complementary chemokine receptors (CCR-1, -2, -3, -5) and other
proinflammatory receptors (toll-like receptor 2 and 4, TNF receptor superfamily
1A and 1B, IL-6R) in PM, with OB group showing less pronounced chemoattracting
and proinflammatory profile compared to T2DM group. In T2DM patients VLCD
decreased body weight, improved metabolic profile, and decreased mRNA
expression of up-regulated CCRs in PM and chemokines [CCL 8, chemokine (C-X-C
motif) ligand 10] in SCAT. VLCD markedly increased mRNA expression of T-
lymphocyte attracting chemokine CCL-17 in SCAT. Conclusion: Obese patients with
and without T2DM have increased mRNA expression of chemotactic and
proinflammatory factors in SCAT and expression of corresponding receptors in
PM. Two weeks of VLCD significantly improved this profile in T2DM patients.

Source: J Clin Endocrinol Metab. 2011 Feb 2. [Epub ahead of print]

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